The Expression of Japanese Encephalitis Virus Envelope Gene in Green Cos

Involvement of the host cell nuclear envelope membranes in the replication of Japanese encephalitis virus.

The distribution of viral ribonucleic acid (RNA) on various cell membrane fractions derived from a porcine kidney cell line infected with Japanese encephalitis virus was investigated. At 40 h postinfection, after virus growth had reached its peak, three viral RNAs, 45S, 27S, and 20S, were associated with the cytoplasmic membranes and intact nuclei. The amount of each RNA associated with the nuc...

Proper maturation of the Japanese encephalitis virus envelope glycoprotein requires cosynthesis with the premembrane protein.

The role of the Japanese encephalitis virus (JEV) premembrane (prM) protein in maturation of the envelope (E) glycoprotein was evaluated by using recombinant vaccinia viruses encoding E in the presence (vP829) or absence (vP658) of prM. Immunofluorescence analyses showed that E appeared to be localized in the endoplasmic reticulum of cells infected with JEV, vP829, or vP658. However, reactivity...

Neuropathogenesis of Japanese encephalitis virus.

In the central nervous system, the Japanese encephalitis virus can replicate only in neurons. The mechanism of the type of neurotropism was analyzed. The susceptibility to Japanese encephalitis virus infection in the rat brain was closely associated with neuronal immaturity. The initial specific binding of the virus to cells is one of the reasons for neurotropism of the Japanese encephalitis vi...

Immunobiology of Japanese Encephalitis Virus

Sequences and Phylogenetic Analysis of Envelope (E) Gene of Iranian Infectious Bronchitis Virus Isolates in Iran

Background and Aims: Infectious bronchitis virus (IBV) is the etiological agent of infectious bronchitis (IB), which is an acute and highly contagious disease of the respiratory and sometimes the urogenital tracts of chickens. In Iran, the disease has been identified in chicken flocks with serological and virus isolation methods. Gene 3c (E) encode envelop (small membrane) protein. For envelope...